Use of tapered amplifier diode laser for biological-friendly high-resolution optical trapping.
نویسندگان
چکیده
A 1064 nm laser is commonly used for biological optical trapping. However, it has the problem of generating reactive oxygen species in the presence of a sensitizer, which leads to photo damage in biological samples. Here we constructed optical tweezers using a tapered amplifier diode laser that operates at 830 nm. Compared to a 1064 nm laser, this laser is friendly to live cells, eliminates photo damage associated with reactive oxygen species, and allows simultaneous two-photon fluorescence imaging of green fluorescent proteins in live mammalian cells. All these advantages could significantly benefit future application of this single molecule technique in biological studies.
منابع مشابه
Dual-wavelength high-power diode laser system based on an external-cavity tapered amplifier with tunable frequency difference
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عنوان ژورنال:
- Optics letters
دوره 35 17 شماره
صفحات -
تاریخ انتشار 2010